Adipose-derived stromal cells differentiate into cholinergic neurons

doi:10.3969/j.issn.2095-4344.2013.01.007

Abstract

Abstract:

BACKGROUND: Adipose-derived stromal cells have multilineage differentiation potential, and it can differentiate into mesodermal cells and endodermal and ectodermal cells. Under certain conditions, they can differentiate into cholinergic neurons.
OBJECTIVE: To explore in vitro culture of adipose-derived stromal cells and their ability to differentiate into cholinergic neurons.
METHODS: Subcutaneous inguinal fat pads from 2-4 week-old Sprague-Dawley rats were taken and then in vitro isolated, cultured and passaged. After induced and cultured with neural induction medium, CD29, CD31 and CD44 expressions was detected by immunocytochemical tests and the phenotypic identification was performed.
RESULTS AND CONCLUSION: During the in vitro culture, adipose-derived stromal cells adhered to culture flask wall, proliferated strongly, and they were positive for CD29 and CD44, but negative for CD31.
After induced and cultured with neural induction medium, the differentiated cells showed a typical neuron-like cells morphology, and the expression of nestin, neuron-specific enolase and microtubule-associated protein 2 was positive. The expression of choline acetyltransferase was positive after induction and differentiation. It indicates that adipose-derived stromal cells can differentiate into neuron-like cells and the differentiated cells have the function of synthesizing choline acetyltransferase.

Key words: stem cells, adipose-derived stem cells, cholinergic neurons, adipose-derived stromal cells, induction, differentiation, rats, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Deng Chun-ying, Liu Bin, Zhang Zhi-yong, Zhang Yu-qin, Li Yan, Yi Hong-li. Adipose-derived stromal cells differentiate into cholinergic neurons[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(1): 44-49.

Figures/Tables 6

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